Hospice Isle of Man received a cheque of £3,304 from the Manx branch of the Freemasons Grand Charity.
All the money was raised by Freemasons and their families in a scheme which has seen £11million given to hospices across Great Britain since 1984.
The donations will go towards helping patients with terminal cancer or life-limiting illnesses.
Isle of Man Freemasons Grand Master Keith Dalrymple says he's thrilled the organisation has continued to provide vital funding for the charity.
"He enjoyed hearing the Manx national anthem!": King greets guests at Government House
His Majesty meets representatives of the Manx community at Garden Party
Crowds gathered in glorious sunshine to see the Monarch
Live blog: King Charles visits the Isle of Man
Updates from the Lord of Mann's first visit to the Island since ascending to the throne
King speaks of Island's 'enduring partnership' with the crown
King Charles III spoke in Tynwald this lunchtime