In their first outing without their talisman Bryn Snellgrove, Douglas went down 33-25 away at Dukinfield.
The game was always likely to be a potential banana skin and saw Douglas slip away from the leading pack at the top of South Lancashire/Cheshire Division Two.
They are now fourth, just nine points clear of fifth placed Vagabonds.
Spirited FC Isle of Man lose out at Padiham
Both sides finish the game with ten men after entertaining contest
Holden 'proud' of FC Isle of Man efforts this season
Ravens heading to Padiham for what could be finale to 25/26 campaign
Rugby: Douglas head to Vale of Lune for play-off challenge
Vagabonds Ladies and Ramsey face final home games of season
Hockey: IoM Women's Over-45's set for EH Championship semi-final
They could become second Island side to reach an EH final this season
Football: 2026 Hospital Cup & Junior Cup competitions to get underway
Preliminary rounds of both tournaments dominate weekend football schedule