In their first outing without their talisman Bryn Snellgrove, Douglas went down 33-25 away at Dukinfield.
The game was always likely to be a potential banana skin and saw Douglas slip away from the leading pack at the top of South Lancashire/Cheshire Division Two.
They are now fourth, just nine points clear of fifth placed Vagabonds.
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Ward, Berg and Parr lead the way
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Castletown stay four points clear atop Division 2
Rugby: Vagabonds Ladies stay top with another victory
Vagabonds men and Douglas also take respective wins on home soil